HT-independent pathway in the export of membrane reporter to the red cell cytoplasm. (A) Live P. falciparum-infected erythrocytes expressing a VTS reporter with the wild type HT signal (SS-RLLYE-GFPmembmyc (top panel) or single point mutation in the HT signal, SS-ALLYE-GFPmembmyc (second panel); SS-RLAYE-GFPmembmyc (third panel) and SSRLLYA- GFPmembmyc (bottom panel). Essentially, all the GFP associated with the wild type RLLYE motif is detected in exported, punctate cleft structures. In contrast R/L/E to A mutant shows different levels of accumulation of GFP within the body of the parasite

Bhattacharjee S, Speicher KD, Stahelin RV, Speicher DW, Haldar K. PI(3)P-independent and -dependent pathways function together in a vacuolar

translocation sequence to target malarial proteins to the host erythrocyte. Mol Biochem Parasitol. 2012 185(2):106-13