Photobleach analysis of PfGAP50-GFP organization. The panels are prebleach images with and without DIC overlay and postbleach images after application of a laser pulse at the positions indicated by arrows. (A) A region of the ER in an early-trophozoite stage parasite shows complete loss of fluorescence at the point of bleaching, partial loss from a connected compartment, and little loss from an adjacent compartment. There was no recovery after 3 min. (B) Application of a laser pulse to PfGAP50-GFP in an apical cap in an early schizont ablates fluorescence with no recovery from adjacent structures. (C) Spot bleaching of a region of PfGAP50-GFP in a region of a mature merozoite results in complete loss of fluorescence at the point of bleaching, with little loss from other regions of the same merozoite or from adjacent merozoites. Bars = 1 μm.

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