The Plasmodium lipocalin PV5 is trafficked to the parasite digestive compartments. PV5 localizes to the central FV, intraparasitic vesicles and to the PV in P. falciparum. Transgenic parasites expressing the endogenous PfPV5 gene fused to mCherry were imaged live in the presence of Lysosensor blue DND-167 (LS Blue). Shown are the signals of mCherry (red, first column), LS Blue (green, second column), a merge of both signals (third column), and DIC images (fourth column). Cyan arrowhead, PfPV5 in PV (Upper). Yellow arrowhead, PfPV5 in small intraparasitic vesicles (Lower). (Scale bars, 5 μm.)

Matz JM, Drepper B, Blum TB, van Genderen E, Burrell A, Martin P, Stach T, Collinson LM, Abrahams JP, Matuschewski K, Blackman MJ. A lipocalin mediates unidirectional heme biomineralization in malaria parasites. Proc Natl Acad Sci U S A. 2020 Jun 29:202001153.