Genetic knockout of GEXP07 led to a loss of PfEMP1 at the RBC surface and impaired adhesion. (A) Wild-type CS2 and GEXP07 knockout-infected RBCs were fixed and probed with anti-PfEMP1 (green) and counter stained with anti-REX1 (red). Nuclei were stained with DAPI (blue). CS2 = parent line. Projections of Z stacks are shown. Scale bar = 5 μm. (B) Whole infected RBCs were treated with trypsin (T) or with trypsin plus trypsin inhibitor (i) and solubilized in Triton X-100, and the resultant pellet representing RBC membrane-embedded PfEMP1 was subjected to Western blotting and probed with anti-ATS. Full-length PfEMP1 (350 kDa) and ATS bands are indicated with blue arrows. Bands at ∼240 kDa represent cross-reaction with spectrin (red arrow). An ∼70-kDa cross-reactive species can be seen in all s McHugh E, Carmo OMS, Blanch A, Looker O, Liu B, Tiash S, Andrew D, Batinovic S, Low AJY, Cho HJ, McMillan P, Tilley L, Dixon MWA. Role of Plasmodium falciparum Protein GEXP07 in Maurer's Cleft Morphology, Knob Architecture, and P. falciparum EMP1 Trafficking. mBio. 2020 PMID: 32184257