The N-terminus of REX2 is important for export. GFP fluorescence in representative live cells of parasites transfected with various REX2GFP constructs. A representation of the modified REX2 is depicted beside each panel of the corresponding cell line; colour coding is as in Fig. 1A, except for the region of GST (blue) and REX3 (purple). Nuclei were stained with DAPI (blue); merges with bright field images (bf)are shown. Deletions REX2d1, d2, d3 and d4 miss amino acid 2–11, 12–21, 22–31 and 32–41 respectively; REX2d5, d6 and d7 miss amino acids 62–72, 73–83 and 84–94 respectively. A. Parasite lines expressing C-terminal REX2GFP deletion mutants (REX2d5–d7 and REX2DC).B. Parasites expressing REX2GFP with deletions in the N-terminus (REX2d1–REX2d4).C. Replacement of the REX2 N-terminus with a sequence from GST (top); replacement of the same sequence with the leader preceding the SP of REX3 is shown in the bottom panel. D. Western blot analysis of protein extracts derived from the transfected parasite lines (indicated above each lane) probed with anti-GFP antibodies. All chimeric proteins appear as single bands of the expected size except for REX2d1 which shows a size similar to REX2DN and af a in ther second band of the expected size. Mass of protein size standard is indicated in kDa.Size bars are 2 mm. Haase S, Herrmann S, Grüring C, Heiber A, Jansen PW, Langer C, Treeck M, Cabrera A, Bruns C, Struck NS, Kono M, Engelberg K, Ruch U, Stunnenberg HG, Gilberger TW, Spielmann T. Sequence requirements for the export of the Plasmodium falciparum Maurer's clefts protein REX2. Mol Microbiol. 2009.