Location of REX2 in acetone fixed IRBCs. (A) Specificity of the anti-REX2 se-rum: anti-REX2 serum reacted only with 3D7(left) but not D10 parasites (right). Merge swith nuclear stain (Hoechst) are shown. (B)IFAs with 3D7 parasites expressing a myc-tagged ETRAMP4 (Spielmann et al., 2006)showed that REX2 (green; Cy2) has a dotty ap-pearance outside of the PVM (ETRAMP4myc in the PVM detected by a rabbit anti-myc antibody; red, Texas Red). No REX2 signal was detectable in D10 parasites expressing ETRAMP4myc (D).(C) REX2 (green; Cy2) colocalized with the Mau-rer’s cleft protein REX1 (Hawthorne et al., 2004)(red; Texas Red) in 3D7 parasites. Bars, 5mm. Haase S, Herrmann S, Grüring C, Heiber A, Jansen PW, Langer C, Treeck M, Cabrera A, Bruns C, Struck NS, Kono M, Engelberg K, Ruch U, Stunnenberg HG, Gilberger TW, Spielmann T. Sequence requirements for the export of the Plasmodium falciparum Maurer's clefts protein REX2. Mol Microbiol. 2009 PMID: 19170882.