Conditional gene disruption confirms essentiality of PI-PLC. Morphological analysis of DMSO- or RAP-treated PLC:HA:loxPint parasites that were allowed to mature to egress-stalled schizonts from 46 to 49 hpi in the presence of C2. parasite morphology was assessed using transmission electron microscopy. Representative images of DMSO- and RAP-treated parasites are displayed in panel I. Results are representative of 18 DMSO- and 30 RAP-treated analyzed parasites. N, nucleus; FV, food vacuole; Rh, rhoptries; H, hemoglobin-containing cytostome. Scale bar, 500 nm.

Burda PC, Ramaprasad A, Bielfeld S, Pietsch E, Woitalla A, Söhnchen C, Singh MN, Strauss J, Sait A, Collinson LM, Schwudke D, Blackman MJ, Gilberger TW. Global analysis of putative phospholipases in Plasmodium falciparum reveals an essential role of the phosphoinositide-specific phospholipase C in parasite maturation. mBio. 2023 Jul 25:e0141323. PMID: 37489900.