Conditional Inactivation of PfRbsn5 leads to vesicles in the parasite and

parasite death. B, Live-cell microscopy images of the indicated stages of PfRbsn5-2xFKBP-GFP-2xFKBPendo parasites (white arrow, nucleus-proximal PfRbsn5endo foci; light blue arrow, faint dispersed signal in the nucleus; dark blue arrow, signal at the food vacuole; purple arrow, intense focus at the food vacuole in schizont stage parasites). C, Live-cell microscopy images of PfRbsn5-2xFKBP-GFP2xFKBPendo parasites, co-expressing Graspepi. White arrows, PfRbsn5endo overlapping or close to GRASP (Golgi marker) foci. D, Live-cell microscopy images of PfRbsn5 (PfRbsn5-2xFKBP-GFP2xFKBPendo+ nmd3’NLS-FRB-mChepi parasites) using a nuclear mislocalizer 1 h after induction of knock-sideway (1h rapalog) compared to the control (control). Sabitzki R, Roßmann AL, Schmitt M, Flemming S, Guillén-Samander A, Behrens HM, Jonscher E, Höhn K, Fröhlke U, Spielmann T. Role of Rabenosyn-5 and Rab5b in host cell cytosol uptake reveals conservation of endosomal transport in malaria parasites. PLoS Biol. 2024 22(5):e3002639. PMID: 38820535