e, Nuclear development in PfCRK4-depleted parasites (D10 parent, representative of three biological replicates, and results with P2G12-PfCRK4-HA-DD). Scale bar, 2 μm. f, Immunofluorescence detection of spindle structures in PfCRK4-depleted parasites (P2G12 parent, representative of two biological replicates). Tub, tubulin. Scale bar, 2 μm. g, Quantification of centriolar plaques by immunofluorescence in PfCRK4-depleted parasites (D10 parent; P value, chi-square test; representative of two biological replicates and results with P2G12-PfCRK4-HA-DD). Cen, centrin. Scale bar, 1 μm. Analysis of nuclei stained with fluorescent DNA-specific dyes confirmed that the nuclei did not divide and revealed a substantially

distorted nuclear morphology (e). Hemispindle structures were evident in PfCRK4-depleted cells (f); however, they were greatly

enlarged relative to spindles in wild-type parasites and might account for the nuclear distortion.

Ganter M, Goldberg JM, Dvorin JD, Paulo JA, King JG, Tripathi AK, Paul AS, Yang J, Coppens I, Jiang RH, Elsworth B, Baker DA, Dinglasan RR, Gygi SP, Duraisingh MT. Plasmodium falciparum CRK4 directs continuous rounds of DNA replication during schizogony. Nat Microbiol. 2017 2:17017