MTRAP Is Expressed in Sexual Stages of P. falciparum (A) Fluorescence microscopy with anti-PfMTRAP (green), anti-Pfg377 (red), and DAPI (blue) in wild-type P. falciparum sexual stages matured in vitro. Stages III, IV, and V gametocytes are shown. BF, brightfield. Scale bar, 5 mm. See also Figure S3. (B) Fluorescence microscopy with anti-PfMTRAP (green), anti-Band3 (red), and DAPI (blue) in wild-type P. falciparum sexual stages matured in vitro. A gametocyte nonactivated (preactivation), a gametocyte activated for 30 s in vitro, and an egressed gamete after 600 s of activation in vitro are shown. BF, brightfield. Scale bar, 5 mm. Bargieri DY, Thiberge S, Tay CL, Carey AF, Rantz A, Hischen F, Lorthiois A, Straschil U, Singh P, Singh S, Triglia T, Tsuboi T, Cowman A, Chitnis C, Alano P, Baum J, Pradel G, Lavazec C, Ménard R. Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes. Cell Host Microbe. 2016 PMID: 27832590