Expression and subcellular localization of PfC3AP2 in P. falciparum. (A,B) Immunofluorescence assay of PfC3AP2, visualized with anti-HA, in asexual blood stage (A) and in gametocytes (B). (C,D) Immunofluorescence assay of PfC3AP2 visualized with anti-HA and co-stained with Mito Tracker-Deep-Red showing co-localization in both asexual (C) and sexual (D) blood stage parasites. Scale bar: 5 µm. (E) Western blot showing the expression of the PfC3AP2 across the different developmental stages of the expression and subcellular localization of PfC3AP2 in P. falciparum. (A,B) Immunofluorescence assay of PfC3AP2, visualized with anti-HA, in asexual blood stage (A) and in gametocytes (B). (C,D) Immunofluorescence assay of PfC3AP2 visualized with anti-HA and co-stained with Mito Tracker-Deep-Red showing co-localization in both asexual (C) and sexual (D) blood stage parasites. Scale bar: 5 µm. (E) Western blot showing the expression of the PfC3AP2 across the different developmental stages of the asexual blood stage cycle. The membranes were probed with an anti-HA antibody, and anti-aldolase was used as a loading control. Sheokand PK, Pradhan S, Maclean AE, Mühleip A, Sheiner L. Plasmodium falciparum Mitochondrial Complex III, the Target of Atovaquone, Is Essential for Progression to the Transmissible Sexual Stages. Int J Mol Sci. 2024 25(17):9239. PMID: 39273187