PfHAD1 is expressed in blood-stage parasites and is localized to the parasite cytoplasm. Immunofluorescence confocal microscopy of ACPLGFP trophozoite and schizont, stained with aGFP and aPfHAD1 antibodies and Hoechst 33258 nuclear stain. Scale bars, 2 mm. Lower panel: PfHAD1-GFP localization is similar to that of PfHAD1 (Upper panel). Shown is live microscopy of AM1 Hsp110:PfHAD1-GFP trophozoite and schizont, stained with Hoechst 33258 nuclear stain. Scale bars represent 2 μm.

Guggisberg AM, Park J, Edwards RL, Kelly ML, Hodge DM, Tolia NH, Odom AR. A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway in malaria parasites. Nat Commun. 2014 24;5:4467.