Localization of PfDyn2 across the asexual blood stage. A, Immunofluorescence assays (IFA) showing localization of PfDyn2 to the apicoplast in the schizont stage, but not in the ring or trophozoite stages. DAPI stains nucleus. PfDyn2 was detected by α-HA and FITC-labeled secondary antibody. The apicoplast was labeled with α-ACP, acyl carrier protein, and TRITC-labeled secondary antibody. B, Immunofluorescence assays (IFA) showing localization of PfDyn2 to the mitochondrion in the schizont stage, but not in the ring or trophozoite stages. DAPI stains nucleus. PfDyn2 was detected by α-HA and FITC-labeled secondary antibody. The mitochondrion was labeled with MitoTracker Red CMXRos (60 nM). Morano AA, Xu W, Shadija N, Dvorin JD, Ke H. The dynamin-related protein Dyn2 is essential for both apicoplast and mitochondrial fission in Plasmodium falciparum. bioRxiv [Preprint]. 2024 03.15.585229. PMID: 38559241