Localization of PfDyn2 across the asexual blood stage. A, Immunofluorescence assays (IFA) showing localization of PfDyn2 to the apicoplast in the schizont stage, but not in the ring or trophozoite stages. DAPI stains nucleus. PfDyn2 was detected by α-HA and FITC-labeled secondary antibody. The apicoplast was labeled with α-ACP, acyl carrier protein, and TRITC-labeled secondary antibody. B, Immunofluorescence assays (IFA) showing localization of PfDyn2 to the mitochondrion in the schizont stage, but not in the ring or trophozoite stages. DAPI stains nucleus. PfDyn2 was detected by α-HA and FITC-labeled secondary antibody. The mitochondrion was labeled with MitoTracker Red CMXRos (60 nM). A-B, images were taken from a Nickon Ti microscope and processed by Nikon NIS elements software. Charneau S, Bastos IM, Mouray E, Ribeiro BM, Santana JM, Grellier P, Florent I. Characterization of PfDYN2, a dynamin-like protein of Plasmodium falciparum expressed in schizonts. Microbes Infect. 2007 9:797-805. PMID: 17533148