U-ExM of PfDyn2-3HAapt parasites comparing segmentation in PfDyn2-sufficient and deficient conditions. Late schizont stage parasites were pretreated with E64 to prevent egress. Maximum projections of Z-stack images (10-20 slices) are shown. The far-right column contains a diagram to help visualize the size and shape of each visible basal complex in the parasite; in the PfDyn2-deficient condition, amitochondrial merozoites are separated from the residual body but merozoites that contain mitochondria are stuck together. The IMC (α-PfIMC1g) is in yellow, the mitochondria (MitoTracker orange CMTMRos) are in in magenta, and nuclei (SYTOX) are blue. AF405 NHS Ester was used as a general protein stain and is shown in grey. Morano AA, Xu W, Shadija N, Dvorin JD, Ke H. The dynamin-related protein Dyn2 is essential for both apicoplast and mitochondrial fission in Plasmodium falciparum. bioRxiv [Preprint]. 2024 2024.03.15.585229. PMID: 38559241