The PfRON4 and PfRON5 proteins localize to the rhoptry neck. A, antibodies raised against PfRON4 and PfRON5 recognize specific protein products in schizont parasite extracts. B, immunofluorescence assays using the anti-PfRON antibodies reveal staining of the apical tip of free merozoite, in close apposition with the rhoptry bulb markers PfRAP1 and PfRAMA,suggesting a rhoptry neck localization. Scale bar: 0.2 m. C, immunoelectron microscopy confirms that PfRON4 localizes to the rhoptry neck. Scale bar: 0.1 m. Richard D, MacRaild CA, Riglar DT, Chan JA, Foley M, Baum J, Ralph SA, Norton RS, Cowman AF. Interaction between Plasmodium falciparum apical membrane antigen 1 and the rhoptry neck protein complex defines a key step in the erythrocyte invasion process of malaria parasites. J Biol Chem. 2010 285(19):14815-22. PMID: 20228060;

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