RON11 is essential for intraerythrocytic growth. (E) IFAs showing the localization of RON11 in RON11apt schizont with respect to 2 rhoptry markers: RON4 (neck) and RAP1 (bulb). Images from left to right are phase-contrast, DAPI (nucleus, cyan), anti-HA (magenta), anti-RON4, or RAP1 (yellow), and fluorescence merge. Z stack images were deconvolved and projected as a combined single image. Representative images of 3 biological replicates. (F) Quantification of the colocalization of RON11apt with RON4 and RAP1 as determined by the PCC (n= 3 biological replicates, 4 schizonts per replicate, the underlying data can be found in S1 Data). Error bars = SEM;****p< 0.0001 by unpaired two-tailedttest. HA, hemagglutinin; PCC, Pearson’s correlation coefficient; SD, standard deviation; SEM, standard error of the mean. Anaguano D, Adewale-Fasoro O, Vick GW, Yanik S, Blauwkamp J, Fierro MA, Absalon S, Srinivasan P, Muralidharan V. Plasmodium RON11 triggers biogenesis of the merozoite rhoptry pair and is essential for erythrocyte invasion. PLoS Biol. 2024 22(9):e3002801. PMID: 39292724;