Immunofluorescence and phase contrast images of late schizonts or free merozoites (insets) to colocalise PfRh5 with RON4. Each panel from left top right corresponds to anti-HA antibodies (to detect PhRh5), rabbit anti-RON4, overlay of each with DAPI nuclear stain, phase contrast and overlay of all images. Insets show individual merozoites. Scale bars = 1 mM. RON4 associates with PfRh5 which localises to the rhoptries in merozoites and follows the tight junction during the process of erythrocyte invasion.
Baum J, Chen L, Healer J, Lopaticki S, Boyle M, Triglia T, Ehlgen F, Ralph SA, Beeson JG, Cowman AF. Reticulocyte-binding protein homologue 5 - An essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum. Int J Parasitol. 2009 39:371-80.