The effect of the absence of GP1 and GP2 on some organelle markers observed by IFA. Immunofluorescence assay on wildtype parasites (GP1-3HA-glmS 0mM GlcN) and on parasites lacking both proteins (KO GP2 in GP1-3HA-glmS 2.5mM GlcN). Markers for different parasite organelles were tested in combination with an anti-HA. Scale bar=5 µm. Investigation by IFA whether the localisation of different organellar markers was affected in the KD/KO line. Only some minor effects were noticeable for the microneme markers AMA1 and EBA175, the inner membrane complex protein GAP45 and the merozoite surface protein MSP1 Globally, the lack of any obvious specific defect might suggest that general parasite fitness is affected.

Hallée S, Theriault C, Gagnon D, Kehrer J, Frischknecht F, Mair GR, Richard D.Identification of a Golgi apparatus protein complex important for the asexual erythrocytic cycle of the malaria parasite Plasmodium falciparum. Cell Microbiol.2018 Mar 26:e12843.