PF3D7_1117500 tyrosine--tRNA ligase

Cellular localization of PfTyrRS. (a) Confocal microscopy of P. falciparum asexual stages—rings, trophozoites and schizonts using anti-sera raised in rabbits against PfTyrRSs. scale bar in red corresponds to a size of 5 μm (b) Confocal microscopy using anti-GFP antibody (green) in PfTyrRSs-GFP transgenic parasite cell lines. Parasite nucleus was stained with DAPI (blue) in each case. Scale bar in red corresponds to a size of 5 μm. Localization data show that PfTyrRS is present in all three stages of asexual parasite life cycle: rings, trophozoites and schizonts. Closer examination of PfTyrRS localization in infected erythrocytes during trophozoite and schizont stages revealed possible secretion of PfTyrRS into infected red blood cell (iRBC), despite absence of an export signal in PfTyrRS sequence (a). Similar results were obtained using PfTyrRS-GFP fusion parasite transgenic cell lines (b).

Bhatt TK, Khan S, Dwivedi VP, Banday MM, Sharma A, Chandele A, Camacho N, Ribas de Pouplana L, Wu Y, Craig AG, Mikkonen AT, Maier AG, Yogavel M, Sharma A. Malaria parasite tyrosyl-tRNA synthetase secretion triggers pro-inflammatory responses. Nat Commun. 2011 2:530.

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