Immuno fluorescence assay demonstrating expression of HA-tagged endogenous PfSR1 during different stages of IDC. Scale bar, 2μm. we used the CRISPR / cas9 system to create a transgenic line in which the 3’ terminus of the endogenous open reading frame is replaced and fused with an HA epitope tag and the glmS ribozyme. This parasite line enables to quantify and visualize the endogenous PfSR1 as well as to induce expression knock-down by adding glucosamine (GlcN) to the culture media.

Goyal M, Singh BK, Simantov K, Kaufman Y, Eshar S, Ron D. An SR protein is essential for activating DNA repair in malaria parasites. J Cell Sci. 2021 jcs.258572.