Development of parasites expressing or lacking PV6 during the ring stage and PV6 localization in schizonts. Costaining of schizonts with antibodies against PV6 (green) and the apical organelle markers AMA1 (micronemes), RAP1 (rhoptry), RON4 (rhoptry neck), and RESA (dense granule) (all in red) by immunofluorescence microscopy. DNA was stained with Hoechst 33342 (blue). Individual channels are shown in Fig. S1B. The scale bar represents 5 µm. Fréville A, Ressurreição M, van Ooij C. Identification of a non-exported Plasmepsin V substrate that functions in the parasitophorous vacuole of malaria parasites. mBio. 2024 15(1):e0122323.