RASP2 plays an essential role in rhoptry secretion. c IFA on E64 or DMSO-treated iKO-PfRASP2-HA3 parasites ± rapamycin. PfRASP2 depletion does not affect PfAMA1 relocalisation at the surface of merozoites (green arrows). f Left: IFA showing two representative examples of a rhoptry secretion event in P. falciparum (RAP2 staining). Right: Quantification of secretion events in iKO-PfRASP2 parasites (±rapamycin). Means ± SD for three independent experiments where >2,500 RBCs were analysed. g Ultrastructure of KD-TgRASP2 + ATc tachyzoites

shows no defect in rhoptry positioning. m: micronemes; c: conoid. Lindström M, Chen L, Jiang S, Zhang D, Gao Y, Zheng J, Hao X, Yang X, Kabbinale A, Thoma J, Metzger LC, Zhang DY, Zhu X, Liu H, Gustafsson CM, Burmann BM, Winderickx J, Sunnerhagen P, Liu B. Lsm7 phase-separated condensates trigger stress granule formation. Nat Commun. 2022 13(1):3701. PMID: 35764627