Image of mNeonGreen (mNG) tagged NBPXa and DBPα schizonts. Scale bars = 5 μm. b Immunofluorescence assay showing HA tagged NBPXa, AMA-1, and RON2 (red), detected in schizonts using an anti-HA antibody. DBPα-mNG detected in same parasites (green) with an anti-mNG antibody. White boxes depict regions enlarged for ‘inset’ panels. c Extended depth of focus images of NBPXa-mNG and DBPα-mNG in post egress merozoites. Scale bars for (b) and (c) = 2 μm.

Hart MN, Mohring F, DonVito SM, Thomas JA, Muller-Sienerth N, Wright GJ, Knuepfer E, Saibil HR, Moon RW. Sequential roles for red blood cell binding proteins enable phased commitment to invasion for malaria parasites. Nat Commun. 2023 14(1):4619. PMID: 37528099