Conditional knockdown of PfPMRT1 has no effect during gametocyte development. Live cell microscopy of 3D7-iGP-PfPMRT1-GFP stage I – V

gametocytes. Scale bar, 2 μm. Conditional knockdown of PfPMRT1 via the glmS ribozyme system resulted in a reduction in PfPMRT1-GFP fluorescence intensity of 79.4% (+/- SD 9.2%) at 7 days post induction (dpi) or 75.5% (+/- SD 23.2%) at 10 dpi, without an effect on gametocyte development