DiQ-BioID reveals proteins in close proximity of PfAIP. (A) Relocalization of BirA-FRB-mcherry (red) to the AIP (green) localization upon addition of rapalog Nuclei stained DAPI. Scale bar 1μm (B) PfAIP based biotinylation at the rhoptries. MeOH fixed schizonts expressing PfAIP-FKBP-GFP (mouse anti-GFP, green). Biotinylation is visualized by streptavidin-594 (red). DAPI was used for DNA staining. Efficient re-localization of the BirA-FRBmCherry construct to the rhoptries was confirmed by live microscopy (A). Additionally, using fluorescently labeled streptavidin we visualized PfAIP-based biotinylation at the rhoptries in the presence of rapalog (B).

Geiger M, Brown C, Wichers JS, Strauss J, Lill A, Thuenauer R, Liffner B, Wilcke L, Lemcke S, Heincke D, Pazicky S, Bachmann A, Löw C, Wilson D, Filarsky M, Burda PC, Zhang K, Junop M, Gilberger TW. Structural insights into PfARO and characterization of its interaction with PfAIP. J Mol Biol. 2019 PMID: 31877322.

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