KIC12 shows a dual localisation in the nucleus and at the K13 compartment and is involved in endocytosis but not in ART resistance. (A) Localisation of

KIC12-2xFKBP-GFP-2xFKBP expressed from the endogenous locus by live-cell microscopy across the intra-erythrocytic development cycle. Arrow heads indicate foci (white) and nuclear (light blue) signal. Nuclei were stained with Hoechst. Scale bar, 5 μm. (B) Live cell microscopy images of parasites expressing KIC12-

2xFKBP-GFP-2xFKBP with episomally expressed mCherry-K13. Scale bar, 5 μm. Black arrowheads indicate overlapping foci. Nuclei were stained with DAPI. Scale

bar, 5 μm. Extended panel shown in S4A Fig (D) Relative growth of asynchronous KIC12-2xFKBP-GFP-2xFKBPendo+1xNLSmislocaliser (blue) and KIC12-2xFKBP-GFP-2xFKBPendo+Lyn-mislocaliser (red) parasites plus rapalog compared with the corresponding untreated control parasites over five days. Five independent growth experiments were performed and mean relative parasitemia +/- SD is shown . Schmidt S, Wichers-Misterek JS, Behrens HM, Birnbaum J, Henshall IG, Dröge J, Jonscher E, Flemming S, Castro-Peña C, Mesén-Ramírez P, Spielmann T. The Kelch13 compartment contains highly divergent vesicle trafficking proteins in malaria parasites. PLoS Pathog. 2023 19(12):e1011814. PMID: 38039338