Location of Pfdyn1 in P. falciparum-infected erythrocytes by immunofluorescence microscopy. Percoll-synchronized late stage cells were smeared onto coverslips and fixed in acetone for 10 min at room temperature. The smears were probed with purified rabbit anti-Pfdyn1-C antibody (1:300) and rat anti-PfGRP antiserum (1:500). Binding of rabbit antibodies was visualized using goat anti-rabbit IgG conjugated with rhodamine. Binding of rat antibodies was visualized using goat anti-rat IgG conjugated with FITC. Coverslips were viewed under Leica TCS NT laser confocal scanning microscope. (A) FITC fluorescent; (B) rhodamine fluorescent; and (C) merged. The location of Pfdyn1 partially overlapped with that of PfGRP PFI0875w, an ER-resident protein. There iss a partial overlap of intracellular localization between Pfdyn1 and PfGRP.

Li H, Han Z, Lu Y, Lin Y, Zhang L, Wu Y, Wang H. Isolation and functional characterization of a dynamin-like gene from Plasmodium falciparum. Biochem Biophys Res Commun. 2004 320:664-71. Copyright Elsevier 2011.