Analysis of expression and localization of Maurer's cleft–associated proteins in IRBCs. (A) Confocal immunofluorescence analysis of resident (A; REX, SBP1, and Pf332) or transiently associated (B; KAHRP and PfEMP1) Maurer's cleft proteins in normal (3D7) SBP1-deleted (1G8) and SBP1-complemented (1G8/sbp1) IRBCs. (C) Colocalization of the resident Maurer's cleft marker MAHRP with PfEMP1 in streptolysin O–pretreated IRBCs demonstrates that PfEMP1 is associated with Maurer's clefts in both 3D7 and SBP1-deteted parasites. Cooke BM, Buckingham DW, Glenister FK, Fernandez KM, Bannister LH, Marti M, Mohandas N, Coppel RL. A Maurer's cleft-associated protein is essential for expression of the major malaria virulence antigen on the surface of infected red blood cells. J Cell Biol. 2006. PMID: 16520384