Trafficking of RIFINS and PfEMP1 in the cytosol of P. falciparum-infected erythrocytes. Immunofluorescence studies were performed on air-dried monolayers of either (A) clinically isolated parasites (here the isolate U12 is shown) cultured in the patient’s own blood, or (B) cultures of parasites adapted to in vitro growth (clone 3D7S8(K+)). Cell monolayers of 18–24 h trophozoite stage IE were probed with antibodies to the conserved C-terminal ends of RIFINS and PfEMP1 as described in Section 2. The dashed line outlines the erythrocyte membrane. Scale bar: 1 μm. Haeggström M, Kironde F, Berzins K, Chen Q, Wahlgren M, Fernandez V. Common trafficking pathway for variant antigens destined for the surface of the Plasmodium falciparum-infected erythrocyte. Mol Biochem Parasitol. 2004. PMID: 14668007

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