Phenotype of the transgenic NF54VAR2CSAS1068A line. (A) VAR2CSA transcriptional profile of S1068A shown by qPCR. Transcriptional levels of each var genes were normalized with the housekeeping gene, seryl-tRNA transferase. (B) Flow cytometry analysis of wild-type and S1068A NF54CSA IEs. IEs were labelled with Rabbit anti-VAR2CSA antibodies. Geometric means of fluorescence intensities and percentage of IEs expressing VAR2CSA are indicated. (C) VAR2CSA immunofluorescence assays (IFA). IFA staining was performed on live cells and fixed and permeabilized smears on wild-type NF54CSA IEs and transgenic NF54VAR2CSAS1068AIEs with rabbit anti-VAR2CSA, anti-GPA and anti-SBP1 antibodies. (D) Western Blot analysis with a goat anti-VAR2CSA antibody was performed on total lysates of wild-type NF54CSA and NF54VAR2CSA S1068AIEs (Upper panel). An anti-PfNapL (PfNucleosome Assembly protein L) serum was used as a loading control for both lysates (Lower panel). Dorin-Semblat D, Semblat JP, Hamelin R, Srivastava A, Tetard M, Matesic G, Doerig C, Gamain B. Casein Kinases 2-dependent phosphorylation of the placental ligand VAR2CSA regulates Plasmodium falciparum-infected erythrocytes cytoadhesion. PLoS Pathog. 2025 PMID: 39804934.