Phenotype of the transgenic NF54VAR2CSAS1068A line. VAR2CSA immunofluorescence assays (IFA). IFA staining was performed on live cells and fixed and permeabilized smears on wild-type NF54CSA IEs and transgenic NF54VAR2CSA S1068AIEs with rabbit anti-VAR2CSA, anti-GPA and anti-SBP1 antibodies. (D) Western Blot analysis with a goat anti-VAR2CSA antibody was performed on total lysates of wild-type NF54CSA and NF54VAR2CSA S1068AIEs (Upper panel). An anti-PfNapL (Pf Nucleosome Assembly protein L) serum was used as a loading control for both lysates (Lower panel). Dorin-Semblat D, Semblat JP, Hamelin R, Srivastava A, Tetard M, Matesic G, Doerig C, Gamain B. Casein Kinases 2-dependent phosphorylation of the placental ligand VAR2CSA regulates Plasmodium falciparum-infected erythrocytes cytoadhesion. PLoS Pathog. 2025