Localization of ISN1 in Plasmodium. a Indirect immunofluorescence microscopy with anti-PfISN1 antibody on intraerythrocytic asexual (3D7 strain) and sexual (3D7A strain) stages of P. falciparum. The absence of signal from pre-immune control ruled out non-specific binding. The antibody used was generated as described in “Methods”. Gametocytes were enriched as described in “Methods”. The experiment was repeated at least three times. Live-cell fluorescence imaging of episomally expressed PfISN1-GFP fusion protein in 3D7_PfISN1-GFP (b) and PbANKA_PfISN1-GFP (c) parasites. Imaging of PbANKA_PfISN1-GFP was performed at least three times with parasites collected from independent mice, and imaging of 3D7_PfISN1-GFP was performed at least three times with parasites harvested from independent cultures. Scalebars correspond to 1 μm. The stages indicated correspond only to (a). Source data are provided as a Source data file. Byun S, Park C, Suh JY, Witte CP, Rhee S. Structure, cooperativity and inhibition of the inosine 5'-monophosphate-specific phosphatase from Saccharomyces cerevisiae. FEBS J. 2024 291(9):1992-2008.