GFP targeting by mutant ACP transit peptides. (A) Apicoplast targeting of the wild-type ACP

leader. (B) Cytosolic targeting of a construct lacking a signal peptide. (C) Secretion to the

parasitophorous vacuole of a construct lacking a transit peptide. The substitutions introduced into

mutants (D to I) are detailed in the accompanying panels at right (25), where a dot ( *) indicates

no change. Check marks indicate putative Hsp70 binding sites. Predictions by the apicoplast protein

predictor PlasmoAP are shown for each mutant. Internal labeling in (F) to (I) likely represents GFP

in the food vacuole that has been taken up from the parasitophorous vacuole during phagocytosis. Foth BJ, Ralph SA, Tonkin CJ, Struck NS, Fraunholz M, Roos DS, Cowman AF, McFadden GI. Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum. Science. 2003 31;299(5607):705-8 PMID: 12560551.