(B) Effect of 2-BMP (2-bromohexadecanoic acid) on PfGAP45 localization. Late schizonts (44–48 hr after invasion) were treated with 50 mM 2-BMP, and PfGAP45 targeting to the inner membrane complex (IMC) was assessed with anti-PfGAP45 antibodies in immunofluorescence assays. DMSO treated parasites were used as a control. Scale bars represent 1 mm. (D) Mutation of the PfGAP45 N-terminal palmitoylation site does not prevent IMC targeting. Anti-HA and anti-PfGAP45 antisera were used to follow localization of tagged and endogenous GAP45 by immunofluorescence in late schizont stage PfGAP45-HA and PfGAP45-Npal-HA expressing parasites. Scale bars represent 1 mm.

Jones ML, Collins MO, Goulding D, Choudhary JS, Rayner JC. Analysis of protein palmitoylation reveals a pervasive role in Plasmodium development and pathogenesis. Cell Host Microbe. 2012 12(2):246-58.