IMC formation is disrupted in the second IDC after indolmycin treatment. Shown are representative images of immunofluorescence assays using anti-GAP45 (1:1,000). Untreated, indolmycin (50 μM) treated and indolmycin

treated with polyprenol rescue (5 μMGGOH) as indicated, collected in the second IDC after treatment. The IMC marker GAP45 localises atypically in indolmycin treated parasites. GGOH supplementation restores GAP45

localisation to the inner membrane complex equivalent to untreated. GAP45, green signal; DAPI, parasite nuclei, blue signal; merge of green and blue signal. Scale bar = 5 μm. BF, bright field; DAPI, 40,6-diamidino-2-phenylindole; GGOH, geranylgeraniol; IDC, intraerythrocytic developmental cycle; IMC, inner membrane complex.

Kennedy K, Cobbold SA, Hanssen E, Birnbaum J, Spillman NJ, McHugh E, Brown H, Tilley L, Spielmann T, McConville MJ, Ralph SA. Delayed death in the malaria parasite Plasmodium falciparum is caused by disruption of prenylation-dependent intracellular trafficking. PLoS Biol. 2019 17(7):e3000376.