Lsm7 co-phase separates with Pab1 in vitro. a Purified Lsm7-GFP and Pab1-RFP phase separate in vitro with the addition of 20% Dextran 70 (pH 7.4, NaCl 150 mM, protein concentration 5 μM). Images show representative data from three independent experiments. Scale bar, 2 μm. b Lsm7-GFP and Pab1-RFP co-phase separate when added together (20% Dextran 70, pH 7.4, NaCl 150 mM, protein concentration 5 μM). Images show representative data from three independent experiments. Scale bar, 2 μm. c The addition of 0.5 μM Pab1 together with 5 μM Lsm7 results in an increased droplet formation (20% Dextran 70, pH 7.4, NaCl 150 mM). Images show representative data from three independent experiments. Scale bar, 2 μm. d Addition of total yeast RNA (200 ng/μl) enhances Lsm7-GFP droplet formation and decreases Pab1-RFP demixing (20% Dextran 70, pH 7.4, NaCl 150 mM, protein concentration 5 μM). Images show representative data from three independent experiments. Scale bar, 2 μm. e During co-phase separation of Lsm7 and Pab1 (5 μM), in the presence of total RNA (200 ng/μl), Lsm7 can rescue the demixing of Pab1 (20% Dextran 70, pH 7.4, NaCl 150 mM). Images show representative data from three independent experiments. Scale bar, 2 μm. Lindström M, Chen L, Jiang S, Zhang D, Gao Y, Zheng J, Hao X, Yang X, Kabbinale A, Thoma J, Metzger LC, Zhang DY, Zhu X, Liu H, Gustafsson CM, Burmann BM, Winderickx J, Sunnerhagen P, Liu B. Lsm7 phase-separated condensates trigger stress granule formation. Nat Commun. 2022 13(1):3701. PMID: 35764627