Immunofluorescence and fluorescence microscopy on Plasmodium falciparum HslV (PfHslV)-enhanced yellow fluorescent protein (EYFP) expressing parasites. C and D: fluorescence microscopy using MitoTracker Red CMXRos (red) and Hoechst 33342 (blue). Bar = 2 mm. D10_PfHslV-EYFP late-stage parasites showed a distinct fluorescence pattern reminiscent of an organellar structure. PfHslV-EYFP is located in the plasmodial mitochondrion.
Tschan S, Kreidenweiss A, Stierhof YD, Sessler N, Fendel R, Mordmüller B. Mitochondrial localization of the threonine peptidase PfHslV, a ClpQ ortholog in Plasmodium falciparum. Int J Parasitol. 2010 40:1517-23 Copyright Elsevier 2010