The HOPS/CORVET core subunits are important for intracellular replication. (B) Live-cell fluorescence microscopy images of transgenic parasite (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. (C) Live cell fluorescence microscopy images of transgenic parasite lines expressing endogenous VPS11/16/18-2xFKBP-GFP together with P40PX-mCherry as marker for endosomes and the digestive vacuole membrane. Nuclei were stained with DAPI. DIC, differential interference contrast. Scale bars: 5 μm. Enlarged micrographs (zoom: 650x) of the indicated white boxes are shown to visualise localization of the VPS subunit in relation to the P40PX (DV membrane). (D) Flow cytometry growth curves over four days of the indicated cell lines in absence (control) or presence of rapalog. Dots indicate mean parasitemia of n=4 replicates and error bars indicate SD. Terawaki S, Vasilev F, Moriwaki T, Otomo T. HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis. Sci Rep. 2023 13(1):18734. PMID: 37907479