In silico analysis and expression of PAP2 homologue in malaria parasite, Plasmodium falciparum, PfPAP2. a Domain architecture of PfPAP2. PfPAP2 harbors all the canonical domains of PAP2 superfamily. (f) Detection of PfPAP2 by IFA. i PfPAP2 is expressed throughout the erythrocytic schizogony. The localization of PfPAP2 was investigated in blood stages of P. falciparum by IFA using confocal microscope, Nikon A1. In the ring stage, PfPAP2 (green) was found around the nucleus stained with DAPI (blue). In trophozoites and late schizont stage, PfPAP2 (green) was found to give a honeycomb-staining pattern. g In the schizont and merozoite stage, colocalization of PfPAP2 (green) with PfMSP1 (red), a merozoite surface marker protein, revealed localization of PfPAP2 below cell membrane towards parasite cytosol. Kumar Sah R, Garg S, Dangi P, Ponnusamy K, Singh S. Phosphatidic acid homeostasis regulated by a type-2 phosphatidic acid phosphatase represents a novel druggable target in malaria intervention. Cell Death Discov. 2019. PMID: 31263575