PfTopoVIB and PfSpo11 form the functional holoenzyme in the parasite. (A and B) Indirect immunofluorescence of LS stage-specific parasites shows the distribution of both PfTopoVIB (red) and PfSpo11 (red) in the parasite nucleus and mitochondria. After calculating the average (mean ± standard error of the mean [SEM]) PCC values (n = 25) in each case, PfTopoVIB and PfSpo11 fluorescence showed a moderate correlation (PCC < 0.5) with nuclear stain DAPI (blue) but strong correlation (PCC > 0.8) with Cytc (green). The average MOC for the fraction of red fluorescence of PfTopoVIB overlapping green Cytc was 0.964, and that for the fraction of PfSpo11 fluorescence overlapping green Cytc was 0.938.

Singh P, Tabassum W, Fangaria N, Dey S, Padhi S, Bhattacharyya MK, Arun Kumar K, Roy A, Bhattacharyya S. Plasmodium Topoisomerase VIB and Spo11 Constitute Functional Type IIB Topoisomerase in Malaria Parasite: Its Possible Role in Mitochondrial DNA Segregation. Microbiol Spectr. 2023 11(3):e0498022.