Localization of V1A on secretory organelles in mature schizont-stage parasites.A–F, immuno-EM images of NF54attB-V1A-3HAapt in the mature schizont-stage. The synchronized late trophozoite-stage parasites were treated with 25 nM of ML10 for 14 h to reach the mature schizont-stage. Parasites were then fixed and subjected to immuno-EM analysis. V1A signals were detected on rhoptries (black arrows) and secretory organelles near rhoptries (red arrows). G and H, negative control images with the primary antibody omitted. Bars in A–H, 500 nm. 3HA, triple hemagglutinin epitope; Dv, digestive vacuole; Immuno-EM, immuno-electron microscopy; N, nucleus; PPM, parasite plasma membrane; PVM, parasitophorous vacuolar membrane; R, rhoptries; RBCM, red blood cell membrane; sv, secretory organelles. Shadija N, Dass S, Xu W, Wang L, Ke H. Functionality of the V-type ATPase during asexual growth and development of Plasmodium falciparum. J Biol Chem. 2024 300(9):107608. PMID: 39084459