KIC12 shows a dual localisation in the nucleus and at the K13 compartment and is involved in endocytosis but not in ART resistance.

(A) Localisation of KIC12-2xFKBP-GFP-2xFKBP expressed from the endogenous locus by live-cell microscopy across the intra-erythrocytic development cycle. Arrow heads indicate foci (white) and nuclear (light blue) signal. Nuclei were stained with Hoechst. Scale bar, 5 μm. (B) Live cell microscopy images of parasites expressing KIC12-2xFKBP-GFP-2xFKBP with episomally expressed mCherry-K13. Scale bar, 5 μm. Black arrowheads indicate overlapping foci. Nuclei were stained with DAPI. Scale bar, 5 μm. Extended panel shown in S4A Fig. (C) Live-cell microscopy of knock sideways (+ rapalog) and control (without rapalog) KIC12-2xFKBP-GFP-2xFKBPendo+1xNLSmislocaliser parasites 4 and 16 hours after the induction of knock-sideways by addition of rapalog. Scale bar, 5 μm. Jonscher E, Flemming S, Castro-Peña C, Mesén-Ramírez P, Spielmann T. The Kelch13 compartment contains highly divergent vesicle trafficking proteins in malaria parasites. PLoS Pathog. 2023 19(12):e1011814. PMID: 38039338