Generation of a PfPRP2-RFA tagged parasite line. IFA using anti-HA (red) or epifluorescence of the GFP (green) on the PfPRP2-RFA tagged line shows an identical punctate pattern. Scale bar represents 5 μm. Nuclei of parasites were stain with DAPI (blue). A typical punctate pattern consistent with the proposed apical tip localization was observed for both the anti-HA antibody and the native GFP fluorescence, in line with PfPRP2 being a potential rhoptry protein.

Hallée S, Richard D. Evidence that the Malaria Parasite Plasmodium falciparum Putative Rhoptry Protein 2 Localizes to the Golgi Apparatus throughout the Erythrocytic Cycle. PLoS One. 2015 Sep 16;10(9):e0138626.