PMV conservation and expression. D, (Top) Immunofluorescence micrographs show rabbit a-PfPMV antibodies (Ra-PfPMV, green) label PfPMV in the ER. Colocalizations were performed with mouse a-PfPMV antibodies (Ma-PfPMV, red), shown previously to label PMV in the ER [16]. (Middle) a-HA antibodies (red) label PfPMVHA (Top) and PvPMVHA (Bottom) in the parasite ER. (Bottom) a-HA antibodies (red) label PvPMVHA in the ER, as shown by clocalization with ERC (green). Sleebs BE, Lopaticki S, Marapana DS, O'Neill MT, Rajasekaran P, Gazdik M, Günther S, Whitehead LW, Lowes KN, Barfod L, Hviid L, Shaw PJ, Hodder AN, Smith BJ, Cowman AF, Boddey JA. Inhibition of Plasmepsin V activity demonstrates its essential role in protein export, PfEMP1 display, and survival of malaria parasites. PLoS Biol. 2014 12(7):e1001897.