MyoF is involved in host cell cytosol uptake and associated with the K13 compartment. (A) Localisation of MyoF-2xFKBP-GFP-2xFKBP expressed from the endogenous locus by live-cell microscopy across the intra-erythrocytic development cycle. Nuclei were stained with DAPI. Scale bar, 5 μm. (B) Live cell microscopy images of parasites expressing the MyoF-2xFKBP-GFP-2xFKBP fusion protein with an episomally expressed FV marker P40PX-mCherry. Scale bar, 5 μm. (C) Live cell microscopy images of parasites expressing the MyoF-2xFKBP-GFP-2xFKBP fusion protein with episomally expressed mCherry-K13. Scale bar, 5 μm. (F) Live-cell microscopy of knock sideways (rapalog) and control MyoF-2xFKBP-GFP-2xFKBPendo+1xNLS mislocaliser parasites 1 hour or 22 hours after the induction by addition of rapalog. Scale bar, 5 μm.

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