1. Culture sample showing adjacent trophozoite (T)-infected erythrocytes stained with anti-PfPMT and anti-Pfg27 antibodies. both membrane and cytoplasmic localizations of the native enzyme could be detected in wild-type parasites using anti-PfPMT antibodies, suggesting that the protein might exist in both membrane-associated and soluble forms

2. Immunofluorescence analysis of wild-type, pfpmtΔ and complemented pfpmtΔ+PfPMT parasites expressing PfPMT under the regulatory control of the P. falciparum CAM1 promoter. PfPMT (green), Pfg27 (red), and Hoechst (blue). No Pfg27 labeling of stage-IV or -V gametocytes could be detected in the pfpmtΔ strain, whereas stage-IV and -V gametocytes expressing both Pfg27 and PfPMT were detected in wild-type cultures.

Bobenchik AM, Witola WH, Augagneur Y, Nic Lochlainn L, Garg A, Pachikara N,Choi JY, Zhao YO, Usmani-Brown S, Lee A, Adjalley SH, Samanta S, Fidock DA, Voelker DR, Fikrig E, Ben Mamoun C. Plasmodium falciparum phospho-ethanolamine methyltransferase is essential for malaria transmission. Proc Natl Acad Sci U S A. 2013 110(45):18262-7