Rearrangement of nuclear microtubules coincides with the onset of S-phase and is independent of processive DNA replication. (A) Super-resolution live-cell imaging of microtubule structures (SPY650-magenta) in a nuclear cycle sensor line, where nuclei are marked with mCherry (blue) and nuclear accumulation of PfPCNA1::GFP (green) marks DNA replication; inserts, microtubule signal; scale bars, 1 µm.

Super-resolution live-cell imaging of PfPCNA1::GFP parasites treated with 15 µM APH from 28 hpi onwards and imaged starting at 32 hpi; scale bars, 1 µm. Machado M, Klaus S, Klaschka D, Guizetti J, Ganter M. Plasmodium falciparum CRK4 links early mitotic events to the onset of S-phase during schizogony. mBio. 2023 22:e0077923. PMID: 37345936.