Immunofluorescence microscopy of Maurer’s cleft-resident proteins. Smears of infected RBCs were fixed with acetone/methanol and probed with a series of antibodies recognizing Maurer’s cleft-resident proteins. Each row represents a phase (or DIC) image, a DAPI stain of the parasite nuclei, the immunofluorescence signal and an overlay of this image with the transmission image. The top row of each panel is wild-type3D7; the second row is Pf3D7DM1.A. MAHRP1.B. SBP1.C. REX1.D. MAHRP2. Scale bar = 5 mm.MAHRP1 is essential to traffic PfEMP1 Spycher C, Rug M, Pachlatko E, Hanssen E, Ferguson D, Cowman AF, Tilley L, Beck HP. The Maurer's cleft protein MAHRP1 is essential for trafficking of PfEMP1 to the surface of Plasmodium falciparum-infected erythrocytes. Mol Microbiol. 2008 68(5):1300-14. PMID: 18410498