PF3D7_1414400 serine/threonine protein phosphatase PP1

PfPP1-null parasites are impaired in the rounding-up step. A. Quantification of PV rounding-up by live-microscopy. Left: Still frames of representative egress recordings of PfPP1-iKOdt treated ± RAP. Control parasites (-RAP) were treated with vehicle, 60 μM BAPTA-AM or 1,5 μM C2. The numbersinthe top left corner of each frame refer to time (min:sec). Scale bar: 2 μm. Right: pie charts representing for each condition the percentage of segmented schizonts that experienced PV rounding-up (green) or not (grey). The number of segmented schizonts analyzed per condition:-RAP, DMSO n=262;-RAP,BAPTA-AM n=124;-RAP, C2 n=230; +RAP n=241. (n=5 independent experiments for DMSO). Seveno M, Loubens MN, Berry L, Graindorge A, Lebrun M, Lavazec C, Lamarque MH. The malaria parasite PP1 phosphatase controls the initiation of the egress pathway of asexual blood-stages by regulating the rounding-up of the vacuole. PLoS Pathog. 2025 PMID: 39808636

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